| 质粒名称: | pLenti X2 Puro DEST (w16-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17296 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氯霉素和氨苄霉素 |
| 空载体大小: | 9070 bp |
| 载体类型: | Lentiviral, RNAi ; Destination vector |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti X2 Puro DEST (w16-1) was a gift from Eric Campeau & Paul Kaufman (爱迪基因 plasmid # 17296) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
pLenti X2 Puro DEST (w16-1)载体质粒基本信息
pLenti X2 Puro DEST (w16-1)质粒图谱载体图谱和pLenti X2 Puro DEST (w16-1)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pLenti X2 Puro DEST (w16-1)质粒载体简介
pLenti X2 Puro DEST (w16-1)质粒序列载体序列
LOCUS pLenti X2 Puro DEST (w16-1) 9070 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION 3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR
insertion, Puro.
ACCESSION .
VERSION .
KEYWORDS pLenti X2 Puro DEST (w16-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9070)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pLenti X2 Puro DEST (w16-1)|
FEATURES Location/Qualifiers
source 1..9070
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(350..369)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1652..1671
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1905..1922
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2039..2060
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2075..2105
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2113..2129
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2118..2140
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2137..2153
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2137..2153
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2172..2192
/label=T3
/note="T3 promoter, forward primer"
promoter 2174..2192
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
LTR 2448..2628
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2672..2797
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3290..3523
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4012..4129
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 4183..4771
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4236..4256)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4654..4665)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 4785..5284
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(4820..4843)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5217..5236
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5305..5904
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5305..5324)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5801..5821
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
protein_bind 6085..6209
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(6250..6555)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(6352..6371)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS complement(6897..7556)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 7471..7490
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(7610..7640)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind complement(7665..7789)
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
LTR 7864..8044
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 8116..8237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8153..8172)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8207..8226
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8256..8391
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8318..8337
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8423..8442)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8424..8442)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8452..8469)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8452..8468)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8461..8483)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8613..9068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8700..8719)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8910..8931
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttccagtgtg
4141 gtggaattct gcagatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
4561 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
4741 agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct
4801 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
4861 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
4921 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
4981 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
5041 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
5101 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
5161 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
5221 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
5281 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
5341 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
5401 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
5461 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
5521 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
5581 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
5641 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
5701 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
5761 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
5821 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
5881 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
5941 agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc
6001 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc tagactcgag
6061 cggccgccac tgtgctggat atcaaccact ttgtacaaga aagctgaacg agaaacgtaa
6121 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
6181 tgtaaaacac aacatatcca gtcactatgg tcgacctgca gactggctgt gtataacgga
6241 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
6301 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
6361 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
6421 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
6481 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
6541 aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa
6601 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
6661 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata
6721 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc
6781 acacctcttt ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa
6841 tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc agatctttac
6901 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
6961 aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
7021 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
7081 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
7141 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
7201 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
7261 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
7321 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
7381 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
7441 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
7501 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
7561 gcttccttag ctcctgaaaa tctcgccgga tcctaactca aaatccacac attatacgag
7621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt gactggatat
7681 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
7741 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtt gatatctgca
7801 gaattccacc acactggact agctcactcc caacgaagac aagatctgct ttttgcttgt
7861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggctg cctagggaac
7921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
7981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
8041 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
8101 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
8161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
8221 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
8281 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
8341 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
8401 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
8461 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
8521 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
8581 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
8641 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
8701 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
8761 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
8821 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
8881 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
8941 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
9001 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
9061 tacaatttcc
//
